4HTS
Crystal Structure of Twin Arginine Translocase Receptor- TatC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-25 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 109.780, 109.780, 107.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.911 - 4.000 |
| R-factor | 0.2916 |
| Rwork | 0.288 |
| R-free | 0.32310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.882 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 110.430 | 39.040 | 4.320 |
| High resolution limit [Å] | 4.000 | 12.970 | 4.100 |
| Number of reflections | 5887 | ||
| <I/σ(I)> | 16.8 | ||
| Completeness [%] | 99.8 | 96.3 | 99.8 |
| Redundancy | 7.7 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 28% Jeffamine ED-2001 0.05M HEPES pH 7.5 10% Methyl-2,4-pentanediol (MPD)., VAPOR DIFFUSION, SITTING DROP, temperature 293K |
