4HTO
Crystal structure of the DBD domain of human DNA ligase IV Apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2010-03-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9795 |
Spacegroup name | F 41 3 2 |
Unit cell lengths | 196.236, 196.236, 196.236 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.057 - 2.807 |
R-factor | 0.21 |
Rwork | 0.207 |
R-free | 0.25900 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.105 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.057 | 50.000 | 2.910 |
High resolution limit [Å] | 2.800 | 5.810 | 2.800 |
Rmerge | 0.064 | 0.030 | 0.684 |
Number of reflections | 8420 | ||
<I/σ(I)> | 70.1 | 60 | 6.1 |
Completeness [%] | 99.8 | 100 | 100 |
Redundancy | 22.7 | 19 | 23.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 16 % PEG 3350, 200 mM (NH4)3PO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
2 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 16 % PEG 3350, 200 mM (NH4)3SO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |