4HTM
Mechanism of CREB Recognition and Coactivation by the CREB Regulated Transcriptional Coactivator CRTC2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 76 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 21.481, 57.631, 23.065 |
| Unit cell angles | 90.00, 94.58, 90.00 |
Refinement procedure
| Resolution | 14.410 - 2.000 |
| R-factor | 0.24934 |
| Rwork | 0.248 |
| R-free | 0.28438 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.707 |
| Data reduction software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 2595 | |
| <I/σ(I)> | 22.1 | 14.9 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 100 mM MES, 150 mM zinc acetate, 3 M NaCl and 12% ethanol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
