4HT9
Crystal structure of E coli Hfq bound to two RNAs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 60.590, 38.280, 84.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.310 - 1.800 |
| R-factor | 0.18572 |
| Rwork | 0.184 |
| R-free | 0.22061 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hk9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.431 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER (2.2.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.244 | 30.295 | 1.900 |
| High resolution limit [Å] | 1.800 | 5.690 | 1.800 |
| Rmerge | 0.026 | 0.268 | |
| Number of reflections | 17910 | ||
| <I/σ(I)> | 18.5 | 20.9 | 2.7 |
| Completeness [%] | 98.6 | 98.4 | 99 |
| Redundancy | 6.5 | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 283 | 100 mM NaCl, 100 mM Cacodylate, 12% PEG 8000, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
