4HT8
Crystal structure of E coli Hfq bound to poly(A) A7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.990, 40.710, 100.850 |
Unit cell angles | 90.00, 101.36, 90.00 |
Refinement procedure
Resolution | 98.870 - 1.900 |
R-factor | 0.1917 |
Rwork | 0.190 |
R-free | 0.23140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hk9 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.345 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 98.874 | 31.150 | 1.790 |
High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
Rmerge | 0.037 | 0.214 | |
Total number of observations | 4312 | 24392 | |
Number of reflections | 45603 | ||
<I/σ(I)> | 12.1 | 15.3 | 3.5 |
Completeness [%] | 99.2 | 83.3 | 100 |
Redundancy | 3.6 | 3.4 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 283 | 200 mM NH4Ac, 100 Tris, 26% 2-propanol, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 283K |