4HT7
CO2 concentrating mechanism protein P, CcmP form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9774 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 175.335, 176.204, 200.538 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.412 - 3.301 |
| R-factor | 0.1951 |
| Rwork | 0.195 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.548 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (autoMR) |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.412 | 2.570 |
| High resolution limit [Å] | 3.300 | 2.510 |
| Rmerge | 0.248 | 0.806 |
| Number of reflections | 92592 | |
| <I/σ(I)> | 9.71 | 2.36 |
| Completeness [%] | 98.6 | 96.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 298 | 17% tacsimate, pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
