4HSP
Crystal structure of a DUF3299 family protein (PA4066) from Pseudomonas aeruginosa PAO1 at 2.45 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-20 |
Detector | ADSC Q315 |
Wavelength(s) | 0.979111,0.979338,0.918401 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 119.033, 119.033, 60.972 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.234 - 2.450 |
R-factor | 0.1749 |
Rwork | 0.173 |
R-free | 0.21300 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | XSCALE (March 15, 2012) |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.234 | 2.540 | |
High resolution limit [Å] | 2.450 | 5.250 | 2.450 |
Rmerge | 0.086 | 0.014 | 0.014 |
Number of reflections | 9810 | 1826 | 1784 |
<I/σ(I)> | 17.37 | 43.1 | 1.6 |
Completeness [%] | 99.4 | 99.2 | 96.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2M sodium chloride, 2.0M ammonium sulfate, 0.1M sodium cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |