4HSF

Lysozyme with Arginine at 318K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-10-03
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	78.353, 78.353, 37.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.806 - 1.820
R-factor	0.1945
Rwork	0.193
R-free	0.22480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.059
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.820	1.820
Rmerge	0.043	0.139
Number of reflections	10871
<I/σ(I)>	43	18.232
Completeness [%]	99.7	99.8
Redundancy	8.1	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.8	318	40mg/ml lysozyme, 50mM sodium acetate, 150mM NaCl. Reservoir Solution: 50mM sodium acetate, 1-1.5M NaCl, 300mM arginine , pH 3.8, VAPOR DIFFUSION, HANGING DROP, temperature 318K