4HSB
S. pombe 3-methyladenine DNA glycosylase-like protein Mag2 bound to damaged DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-06-23 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 54.891, 54.891, 153.457 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.855 - 1.900 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.23450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.117 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.152 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 22239 | |
| <I/σ(I)> | 30.3 | |
| Completeness [%] | 99.7 | 98.8 |
| Redundancy | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294.15 | 50 mM HEPES, 200 mM KCl, 25 mM MgSO4 , pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294.15K |
