4HS5
Frataxin from Psychromonas ingrahamii as a model to study stability modulation within CyaY protein family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 177 |
| Detector technology | PIXEL |
| Collection date | 2012-04-02 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9191 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.841, 50.133, 45.748 |
| Unit cell angles | 90.00, 91.35, 90.00 |
Refinement procedure
| Resolution | 31.186 - 1.450 |
| R-factor | 0.1872 |
| Rwork | 0.186 |
| R-free | 0.20160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1EW4 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.128 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.036 | |
| Number of reflections | 31292 | |
| <I/σ(I)> | 26.5 | |
| Completeness [%] | 97.1 | 97.1 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 297 | 20mM sodium acetate, 200mM MgCl2, 29.5% PEG 4000, pH 4.80, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
