4HRX
Crystal structure of KAI2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95390 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.199, 56.037, 52.425 |
| Unit cell angles | 90.00, 116.12, 90.00 |
Refinement procedure
| Resolution | 47.070 - 2.110 |
| R-factor | 0.1632 |
| Rwork | 0.161 |
| R-free | 0.20630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wom |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.040 | 2.230 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.162 | |
| Number of reflections | 14659 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 97.1 | 86.5 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 293 | 1.5M sodium potassium phosphate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
