4HRV
Crystal Structure of Lipoprotein GNA1162 from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.912, 96.130, 44.977 |
| Unit cell angles | 90.00, 112.82, 90.00 |
Refinement procedure
| Resolution | 31.393 - 1.890 |
| R-factor | 0.233 |
| Rwork | 0.232 |
| R-free | 0.25020 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Number of reflections | 22968 | |
| Completeness [%] | 98.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 25% ethylene glycol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
