4HRV
Crystal Structure of Lipoprotein GNA1162 from Neisseria meningitidis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.912, 96.130, 44.977 |
Unit cell angles | 90.00, 112.82, 90.00 |
Refinement procedure
Resolution | 31.393 - 1.890 |
R-factor | 0.233 |
Rwork | 0.232 |
R-free | 0.25020 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.960 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 1.890 | 1.890 |
Number of reflections | 22968 | |
Completeness [%] | 98.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 25% ethylene glycol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |