4HRT
Scapharca tetrameric hemoglobin, unliganded
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 94.353, 100.958, 127.165 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.320 - 1.460 |
| R-factor | 0.13525 |
| Rwork | 0.133 |
| R-free | 0.17492 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hrr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.283 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.450 |
| Rmerge | 0.067 |
| Number of reflections | 197685 |
| <I/σ(I)> | 13.9 |
| Completeness [%] | 92.2 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 5.8 | 298 | 1.9-2.2 Na/K Phosphate, pH 5.8, SMALL TUBES, temperature 298K |
