4HRS
Crystal structure of H. volcanii small archaeal modifier protein 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 24.605, 64.603, 104.555 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.960 - 2.300 |
| R-factor | 0.21682 |
| Rwork | 0.215 |
| R-free | 0.25365 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CS-Rosetta model |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.214 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.960 | 2.040 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.062 | 0.293 |
| Number of reflections | 5594 | |
| <I/σ(I)> | 43.4 | 6.3 |
| Completeness [%] | 95.7 | 97.2 |
| Redundancy | 12.6 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277.15 | 100 mM bis-tris-propane-HCl (pH 6.8), 25-30% (w/v) PEG 400, 0.2 M MgCl2, 0.1 M KCl, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
