4HRF
Atomic structure of DUSP26
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.972, 82.260, 91.678 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.680 |
| R-factor | 0.18659 |
| Rwork | 0.185 |
| R-free | 0.21569 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.897 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.860 |
| High resolution limit [Å] | 1.680 | 1.800 |
| Number of reflections | 55848 | |
| Completeness [%] | 98.0 | 88.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 0.15M Calcium Acetate, 0.1M Na Cacodylate pH 6.5, 17% PEG 8,000, VAPOR DIFFUSION, temperature 298K |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | 0.15M Calcium Acetate, 0.1M Na Cacodylate pH 6.5, 17% PEG 8,000, VAPOR DIFFUSION, temperature 298K |
