4HR1
Structure of PAV1-137, a protein from the virus PAV1 that infects Pyrococcus abyssi.
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 73.546, 73.546, 191.549 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.220 - 2.500 |
R-factor | 0.2343 |
Rwork | 0.232 |
R-free | 0.28030 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.130 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.058 | 0.459 |
Number of reflections | 15911 | |
<I/σ(I)> | 19 | 2.4 |
Completeness [%] | 97.4 | 85.3 |
Redundancy | 5.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291 | 35% PEG400, 0.5M NH4Cl, 0.1M Na citrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |