4HQX
CRYSTAL STRUCTURE OF HUMAN PDGF-BB IN COMPLEX WITH A Modified nucleotide aptamer (SOMAmer SL4)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979176 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.430, 59.430, 168.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.675 - 2.300 |
| R-factor | 0.241 |
| Rwork | 0.239 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CHAIN A of 3MJG |
| RMSD bond length | 0.007 |
| RMSD bond angle | 2.005 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.675 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.064 | 0.572 |
| Number of reflections | 13750 | |
| <I/σ(I)> | 19.29 | 2.6 |
| Completeness [%] | 97.2 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | PROPLEX E1: 0.1 M MAGNESIUM ACETATE, 0.1 M SODIUM ACETATE PH 4.5, 8% PEG 8000, VAPOR DIFFUSION, TEMPERATURE 289K, VAPOR DIFFUSION, SITTING DROP |
