4HQF
Crystal structure of Plasmodium falciparum TRAP, I4 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | I 4 |
| Unit cell lengths | 110.220, 110.220, 47.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.855 - 2.200 |
| R-factor | 0.1752 |
| Rwork | 0.173 |
| R-free | 0.21750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.097 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.270 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 14268 | |
| <I/σ(I)> | 11.26 | 1.69 |
| Completeness [%] | 98.1 | 97.5 |
| Redundancy | 2.89 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 277 | 0.1 M Tris, pH 8.5, 0.2 M lithium sulfate, 25% PEG4000, EVAPORATION, temperature 277K |
