4HQE

The crystal structure of QsrR-DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-03-08
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97872
Spacegroup name	P 41
Unit cell lengths	48.189, 48.189, 156.807
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.815 - 2.299
R-factor	0.208
Rwork	0.205
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.274
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.299
Number of reflections	15818

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.2 M magnesium chloride, 0.1 M Tris.HCl, pH 8.5, 25% (w/v) polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K