4HQD
Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium Target OR265.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97905 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.004, 30.461, 70.061 |
| Unit cell angles | 90.00, 92.68, 90.00 |
Refinement procedure
| Resolution | 34.992 - 2.433 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.22700 |
| Structure solution method | MIR |
| Starting model (for MR) | 4hb5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.169 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.520 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.094 | 0.212 |
| Number of reflections | 20809 | |
| <I/σ(I)> | 17.6 | 4.34 |
| Completeness [%] | 97.9 | 95.1 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch crystallization under oil | 7.5 | 277 | 40% PEG 400, 0.1M lithium sulfate, 0.1M HEPES, pH 7.5, Microbatch crystallization under oil, temperature 277K |
