4HQ8
Crystal structure of a green-to-red photoconvertible DRONPA, pcDRONPA in the green-on-state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 2012-01-15 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 72.378, 107.186, 180.183 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.456 - 1.950 |
R-factor | 0.1866 |
Rwork | 0.184 |
R-free | 0.23510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z1o |
RMSD bond length | 0.009 |
RMSD bond angle | 1.289 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.920 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.096 | 0.676 |
Number of reflections | 101878 | |
<I/σ(I)> | 2.1 | |
Completeness [%] | 99.1 | 99.3 |
Redundancy | 3.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 25%(w/v) PEG 3350, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |