4HPM
PCGF1 Ub fold (RAWUL)/BCORL1 PUFD Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.784, 65.794, 56.517 |
| Unit cell angles | 90.00, 94.73, 90.00 |
Refinement procedure
| Resolution | 19.710 - 1.850 |
| R-factor | 0.2021 |
| Rwork | 0.199 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hpl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.003 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.710 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 32631 | |
| <I/σ(I)> | 16.3 | 2.6 |
| Completeness [%] | 98.8 | 92.8 |
| Redundancy | 4.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295 | 100 mM sodium citrate, 19% polyethylene glycol (PEG) 1500, 100 mM dibasic sodium phosphate., pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
