4HPK
Crystal structure of Clostridium histolyticum colg collagenase collagen-binding domain 3B at 1.35 Angstrom resolution in presence of calcium nitrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2002-11-22 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.98045 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.866, 59.170, 48.800 |
Unit cell angles | 90.00, 100.77, 90.00 |
Refinement procedure
Resolution | 16.610 - 1.350 |
R-factor | 0.18033 |
Rwork | 0.179 |
R-free | 0.21030 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.008 |
RMSD bond angle | 1.222 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC (5.2.0019) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 16.610 | 1.830 | 1.400 |
High resolution limit [Å] | 1.350 | 1.700 | 1.350 |
Number of reflections | 50130 | ||
Completeness [%] | 89.8 | 4.8 | 3.2 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 25-27% PEG 3350, 100mM Sodium acetate, 600mM LiCl, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |