4HPI
Crystal Structure of Hen Egg White Lysozyme complex with GN2-M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-06-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.416, 77.416, 38.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.190 |
| R-factor | 0.16823 |
| Rwork | 0.168 |
| R-free | 0.18037 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bwj |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.211 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.740 | 1.210 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.074 | 0.262 |
| Number of reflections | 37127 | |
| <I/σ(I)> | 76 | 10.3 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 13.8 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2M Magnesium chloride hexahydrate, 0.1M BIS-TRIS(pH6.5), 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
