4HP9
Crystal structure of the N-terminal truncated PAS domain from the hERG potassium channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-18 |
Detector | MARCCD |
Wavelength(s) | 1.00150 |
Spacegroup name | P 42 3 2 |
Unit cell lengths | 89.528, 89.528, 89.528 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.764 - 2.120 |
R-factor | 0.1959 |
Rwork | 0.194 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1byw |
RMSD bond length | 0.008 |
RMSD bond angle | 1.033 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.9) |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 89.528 | 89.528 | 2.230 |
High resolution limit [Å] | 2.120 | 6.700 | 2.120 |
Rmerge | 0.021 | 0.691 | |
Rmeas | 0.023 | 0.783 | |
Rpim | 0.008 | 0.270 | |
Total number of observations | 2417 | 7979 | |
Number of reflections | 7429 | ||
<I/σ(I)> | 21.7 | 62 | 2.9 |
Completeness [%] | 99.5 | 100 | 97.1 |
Redundancy | 9.9 | 8 | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 30% PEG-MME2000, 0.1M potasiumm thiocyanate, 1.0% benzamidine-HCl, vapor diffusion, sitting drop, temperature 298K |