4HP4
Crystal structure of PAS domain from the fruit-fly ELK potassium channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-12-19 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.933 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 150.306, 150.306, 150.306 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.141 - 2.000 |
R-factor | 0.1736 |
Rwork | 0.173 |
R-free | 0.19120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1byw |
RMSD bond length | 0.003 |
RMSD bond angle | 0.742 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.2.25) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 53.149 | 61.362 | 2.110 |
High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
Rmerge | 0.089 | 0.051 | 0.550 |
Number of reflections | 39431 | ||
<I/σ(I)> | 19.5 | 8.4 | 1.4 |
Completeness [%] | 99.5 | 95.3 | 99.4 |
Redundancy | 14.7 | 21.3 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 30% PEG5000, 0.35M ammonium sulfate, 0.1M MES, pH 6.5, vapor diffusion, sitting drop, temperature 277K |