4HOW
The crystal structure of isomaltulose synthase from Erwinia rhapontici NX5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-21 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.856, 81.267, 137.998 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.060 - 1.700 |
| R-factor | 0.1608 |
| Rwork | 0.159 |
| R-free | 0.19339 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m53 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.252 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 99625 | |
| Completeness [%] | 70.0 | 96.6 |
| Redundancy | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 30% PEG 6000, 0.1M Bicine, 10%(w/v) Glycerol, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
