4HOH
RIBONUCLEASE T1 (THR93ALA MUTANT) COMPLEXED WITH 2'GMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1997-11-01 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.320, 60.310, 101.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.050 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rga |
RMSD bond length | 0.007 |
RMSD bond angle | 1.450 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.140 |
High resolution limit [Å] | 2.050 | 2.050 |
Number of reflections | 21739 | |
<I/σ(I)> | 11.9 | 5.25 |
Completeness [%] | 92.8 | 80.1 |
Redundancy | 2.95 | 2.29 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 4.2 | pH 4.20 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 10 (mg/ml) | |
2 | 1 | 1 | 25 (mM) | ||
3 | 1 | 1 | 2'GMP | 2.8 (mM) | |
4 | 1 | 1 | 6.25 (mM) | ||
5 | 1 | 1 | MPD | 42.5 (%) |