4HOD
Crystal structure of LeuT-E290S with bound Cl
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-12-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9191 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.580, 87.480, 80.860 |
Unit cell angles | 90.00, 95.00, 90.00 |
Refinement procedure
Resolution | 47.622 - 3.300 |
R-factor | 0.1952 |
Rwork | 0.190 |
R-free | 0.25410 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.861 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 9091 | |
Completeness [%] | 98.2 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.1-0.2M NaCl, 16-23% PEG 550 MME, 0.1 M Hepes, pH 7.0, 1mM Leu, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.1-0.2M NaCl, 16-23% PEG 550 MME, 0.1 M Hepes, pH 7.0, 1mM Leu, VAPOR DIFFUSION, SITTING DROP, temperature 292K |