4HOB

The crystal structure of the Zalpha domain from Cyprinid Herpes virus 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	51.736, 54.584, 86.642
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.183 - 1.760
R-factor	0.2036
Rwork	0.201
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qbj
RMSD bond length	0.003
RMSD bond angle	0.677
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.183	1.830	1.830
High resolution limit [Å]	1.760	1.770	1.760
Rmerge	0.036
Number of reflections	23721
<I/σ(I)>	13.1	2.3	48.6
Completeness [%]	94.8	76.4	87.7
Redundancy	4.2	3.7	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.2M NaCl, 0.1M Cacodylate, 2M Ammonium Sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K