4HOB
The crystal structure of the Zalpha domain from Cyprinid Herpes virus 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.736, 54.584, 86.642 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.183 - 1.760 |
| R-factor | 0.2036 |
| Rwork | 0.201 |
| R-free | 0.24660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qbj |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.677 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.183 | 1.830 | 1.830 |
| High resolution limit [Å] | 1.760 | 1.770 | 1.760 |
| Rmerge | 0.036 | ||
| Number of reflections | 23721 | ||
| <I/σ(I)> | 13.1 | 2.3 | 48.6 |
| Completeness [%] | 94.8 | 76.4 | 87.7 |
| Redundancy | 4.2 | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M NaCl, 0.1M Cacodylate, 2M Ammonium Sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
