4HNS

Crystal structure of activated CheY3 of Vibrio cholerae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-05-22
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	H 3
Unit cell lengths	67.320, 67.320, 72.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.220 - 2.100
R-factor	0.231
Rwork	0.231
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3to5
RMSD bond length	0.012
RMSD bond angle	1.600
Data reduction software	AUTOMAR
Phasing software	AMoRE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.100	2.100
Number of reflections	6881
Completeness [%]	95.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	10% PEG6000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K