4HNQ
Crystal Structure of the mutant Q97A of Vibrio cholerae CheY3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-02-17 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | H 3 |
Unit cell lengths | 67.640, 67.640, 74.918 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.070 - 2.400 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3to5 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.900 |
Data reduction software | AUTOMAR |
Phasing software | AMoRE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.070 | |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 4141 | |
Completeness [%] | 85.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.6 M ammonium sulfate, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |