4HN9
Crystal structure of iron ABC transporter solute-binding protein from Eubacterium eligens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97923 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.870, 57.564, 79.299 |
Unit cell angles | 90.00, 98.20, 90.00 |
Refinement procedure
Resolution | 30.012 - 1.850 |
R-factor | 0.1509 |
Rwork | 0.150 |
R-free | 0.19820 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.395 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.084 | 0.724 |
Number of reflections | 47253 | |
<I/σ(I)> | 13.8 | 1.98 |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 297 | 35% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |