4HMZ
Crystal Structure of ChmJ, a 3'-monoepimerase from Streptomyces bikiniensis in complex with dTDP-quinovose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-10 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.5418 |
Spacegroup name | I 4 |
Unit cell lengths | 140.721, 140.721, 117.736 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.20165 |
Rwork | 0.199 |
R-free | 0.24883 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c0z |
RMSD bond length | 0.009 |
RMSD bond angle | 2.217 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.500 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.088 | |
Number of reflections | 73347 | |
<I/σ(I)> | 1.95 | |
Completeness [%] | 94.9 | 89.2 |
Redundancy | 4.2 | 1.78 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 20% PEG 3400, 2% 2-methyl-2,4-pentanediol, 100 mM MOPS, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |