4HMT
Crystal structure of PhzG from Pseudomonas fluorescens 2-79 in complex with hexahydrophenazine-1,6-dicarboxylic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.98004 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.593, 63.425, 133.546 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.612 - 1.420 |
R-factor | 0.1175 |
Rwork | 0.116 |
R-free | 0.15300 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.017 |
RMSD bond angle | 1.667 |
Data scaling software | XSCALE |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.000 |
High resolution limit [Å] | 1.420 |
Number of reflections | 89891 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 21-24% (w/v) PEG 3350, 0.2M (NH4)2SO4, 0.1M BisTris, 15uM PhzF, 30uM PhzA/B, 0.2M TRIS-HCl pH 7.5, vapor diffusion, hanging drop, temperature 298K |