4HMI
Crystal structure of Staphylococcal nuclease variant Delta+PHS V99K at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 100 |
Detector technology | FLAT PANEL |
Collection date | 2012-03-17 |
Detector | PHOTON 100 |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.161, 61.124, 38.115 |
Unit cell angles | 90.00, 92.95, 90.00 |
Refinement procedure
Resolution | 19.220 - 1.750 |
R-factor | 0.1899 |
Rwork | 0.188 |
R-free | 0.22090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.019 |
RMSD bond angle | 1.853 |
Data reduction software | APEX |
Phasing software | PHASER (2.2.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.220 | 1.850 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.031 | 0.243 |
Number of reflections | 14469 | |
<I/σ(I)> | 19.14 | 3.7 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6.86 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 20% MPD, 25 mM potassium phosphate, with calcium chloride and pdTp added to protein in a 3:2:1 ratio, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |