4HLB
Crystal structure of an alpha-lytic protease prodomain-like protein (DESPIG_01740) from Desulfovibrio piger ATCC 29098 at 1.80 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979095,0.979338,0.953725 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 57.821, 57.821, 63.890 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.910 - 1.800 |
R-factor | 0.1966 |
Rwork | 0.195 |
R-free | 0.22280 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.361 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.20) |
Phasing software | SOLVE |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.910 | 28.911 | 1.850 |
High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
Rmerge | 0.014 | 0.014 | |
Total number of observations | 1903 | 6715 | |
Number of reflections | 11856 | ||
<I/σ(I)> | 16.3 | 17.4 | 0.6 |
Completeness [%] | 100.0 | 98.3 | 100 |
Redundancy | 13.3 | 11.9 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.20M sodium acetate, 30.00% polyethylene glycol 4000, 0.1M tris hydrochloride pH 8.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |