4HKU
The crystal structure of TetR transcriptional regulator (lmo2814) from Listeria monocytogenes EGD-e
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926, 0.97951 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.668, 81.560, 105.425 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.708 - 2.302 |
| R-factor | 0.1976 |
| Rwork | 0.195 |
| R-free | 0.25670 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.999 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.102 | 0.740 |
| Number of reflections | 18258 | |
| <I/σ(I)> | 26.4 | 2.4 |
| Completeness [%] | 98.9 | 98.1 |
| Redundancy | 7.1 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M Sodium Acetate, 0.1M HEPES:NaOH, 20%(w/v) PEG3000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
