4HKJ
Structure of Cowpox CPXV203 in complex with MHCI (H-2Kb)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-10 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.97909 |
| Spacegroup name | P 1 |
| Unit cell lengths | 88.310, 88.250, 106.420 |
| Unit cell angles | 76.18, 69.29, 66.69 |
Refinement procedure
| Resolution | 49.360 - 3.000 |
| R-factor | 0.2307 |
| Rwork | 0.230 |
| R-free | 0.25300 |
| Structure solution method | SAD, MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2clz |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.538 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.190 |
| High resolution limit [Å] | 3.000 | 5.450 | 3.000 |
| Rmerge | 0.164 | 0.107 | 0.622 |
| Number of reflections | 47882 | ||
| <I/σ(I)> | 10.1 | 10.8 | 2.55 |
| Completeness [%] | 87.9 | 99.7 | 84.9 |
| Redundancy | 4.1 | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.55 | 293 | 10% PEG6000, 4% glucose, 2% ethylene glycol, 0.1 M tri-potassium citrate, 0.01% azide, pH 5.55, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
