4HKB

CH67 Fab (unbound) from the CH65-67 Lineage

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97919
Spacegroup name	P 21 21 21
Unit cell lengths	113.249, 123.269, 228.828
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.698 - 3.600
R-factor	0.2608
Rwork	0.258
R-free	0.30560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.378
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.660
High resolution limit [Å]	3.600	9.750	3.600
Rmerge	0.107	0.039	0.452
Number of reflections	38632
<I/σ(I)>	6.6
Completeness [%]	99.8	98.8	99.9
Redundancy	4.8	4.4	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	15-20% PEG4000, 10 mM Tris-HCl, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K