4HK8
Crystal Structures of Mutant Endo- -1,4-xylanase II Complexed with substrate (1.15 A) and Products (1.6 A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 38.492, 45.526, 111.417 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.570 - 1.151 |
R-factor | 0.1116 |
Rwork | 0.111 |
R-free | 0.12730 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.479 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.150 |
Number of reflections | 63877 |
Completeness [%] | 90.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | 0.2 M Ammonium Citrate tribasic pH7.0, 20% PEG 3350, vapor diffusion, temperature 298K |