Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-07-01 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.8420 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.778, 94.853, 162.016 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.758 - 2.300 |
R-factor | 0.1668 |
Rwork | 0.163 |
R-free | 0.20300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3t36 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.185 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 23.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 54621 |
<I/σ(I)> | 13.1 |
Completeness [%] | 98.2 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M lithium sulfate, 15% PEG8000, 20 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |