4HJ8

Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGTTACGCT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-03-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	44.584, 57.548, 66.575
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.779 - 2.043
R-factor	0.1962
Rwork	0.192
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hik
RMSD bond length	0.007
RMSD bond angle	1.046
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.120
High resolution limit [Å]	2.043	4.420	2.050
Rmerge	0.073	0.029	0.459
Number of reflections	11273
<I/σ(I)>	9
Completeness [%]	99.5	96.4	98.9
Redundancy	3.4	3.3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	35% PEG 8000, 0.2M sodium formate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K