4HJ7
Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGTTAGGGT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-03-08 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.756, 58.181, 65.626 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.512 - 1.783 |
R-factor | 0.1769 |
Rwork | 0.174 |
R-free | 0.20690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hik |
RMSD bond length | 0.007 |
RMSD bond angle | 1.144 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (1.7_650) |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.850 |
High resolution limit [Å] | 1.783 | 3.860 | 1.790 |
Rmerge | 0.036 | 0.024 | 0.192 |
Number of reflections | 15354 | ||
<I/σ(I)> | 18 | ||
Completeness [%] | 99.5 | 97.1 | 99.5 |
Redundancy | 3.5 | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 30% PEG 6000, 0.2M lithium nitrate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K |