4HJ5
Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGTTTCGGT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-02-07 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.873, 59.244, 65.763 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.951 - 2.040 |
R-factor | 0.2295 |
Rwork | 0.226 |
R-free | 0.26090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hik |
RMSD bond length | 0.008 |
RMSD bond angle | 1.097 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (1.7_650) |
Refinement software | PHENIX (1.7_650) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.120 |
High resolution limit [Å] | 2.040 | 4.420 | 2.050 |
Rmerge | 0.077 | 0.045 | 0.411 |
Number of reflections | 9855 | ||
<I/σ(I)> | 10.6 | ||
Completeness [%] | 93.0 | 89.1 | 95 |
Redundancy | 2.4 | 2.2 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 35% PEG 6000, 0.2M sodium formate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K |