4HJ4

Crystal Structure of Rhodobacter Sphaeroides LOV protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Collection date	2010-11-11
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	65.800, 70.500, 74.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.813 - 2.703
R-factor	0.1997
Rwork	0.194
R-free	0.25160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.234
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.750
High resolution limit [Å]	2.700	7.300	2.700
Rmerge	0.107	0.078	0.366
Number of reflections	9927
<I/σ(I)>	11
Completeness [%]	100.0	100	99.2
Redundancy	6.9	6.2	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	290	30% PEG400, 0.15 M MgCl2, 0.1 M HEPES, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K