4HIY
crystal structure of R34/53A, D95N, H112W mutant of borna disease virus matrix protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 137.417, 137.417, 137.417 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.000 - 3.310 |
R-factor | 0.1807 |
Rwork | 0.175 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f1j |
RMSD bond length | 0.010 |
RMSD bond angle | 1.439 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.000 | 3.480 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.155 | 0.789 |
Number of reflections | 3517 | |
<I/σ(I)> | 11.9 | 3.2 |
Completeness [%] | 99.4 | 100 |
Redundancy | 11.2 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.125 Li2SO4, 25% GLYCEROL, 0.075 HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |