4HIW
Crystal structure of R34/53A, H112W mutant of borna disease virus matrix protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 136.804, 136.804, 136.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.2042 |
| Rwork | 0.195 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f1j |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.599 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.107 | 0.731 |
| Number of reflections | 5121 | |
| <I/σ(I)> | 13.6 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.9 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.0 M NaCl, 0.1 M NaH2PO4, 0.1 M KH2PO4, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
