4HIQ
The Structure of V122I Mutant Transthyretin in Complex with AG10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Detector technology | PIXEL |
Collection date | 2012-06-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9797 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 42.727, 84.839, 63.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.910 - 1.180 |
R-factor | 0.1613 |
Rwork | 0.160 |
R-free | 0.18600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fbr |
RMSD bond length | 0.017 |
RMSD bond angle | 1.725 |
Data reduction software | autoXDS |
Data scaling software | autoXDS |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.910 | 1.250 |
High resolution limit [Å] | 1.180 | 1.180 |
Number of reflections | 75242 | |
<I/σ(I)> | 34.5 | 4.5 |
Completeness [%] | 98.6 | 91 |
Redundancy | 6.1 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | THE WT-TTR WAS CONCENTRATED TO 4 MG/ML IN 10 MM NAPI, 100 MM KCL, AT PH 7.6 AND CO-CRYSTALLIZED AT ROOM TEMPERATURE WITH INHIBITORS USING THE VAPOR-DIFFUSION SITTING DROP METHOD, CRYSTALS WERE GROWN FROM 1.395 M SODIUM CITRATE, 3.5% V/V GLYCEROL AT PH 5.5. THE CRYSTALS WERE FROZEN USING A CRYO-PROTECTANT SOLUTION OF 1.395 M SODIUM CITRATE, PH 5.5, CONTAINING 10% V/V GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 298K |