4HIL
1.25A Resolution Structure of Rat Type B Cytochrome b5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.609, 67.847, 70.342 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.171 - 1.250 |
| R-factor | 0.1379 |
| Rwork | 0.137 |
| R-free | 0.15220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mus |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.328 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.3_927) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.833 | 1.320 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 51942 | |
| <I/σ(I)> | 13.4 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 30% (w/v) PEG 8000, 100 sodium cacodylate, 200 mM sodium acetate, pH 6.5, vapor diffusion, temperature 293K |
